Limonoid Extracted from Chisocheton macrophyllus Seeds and Their Cytotoxic Activity against MCF-7 Breast Cancer Cell Lines

Limonoid Extracted from Chisocheton macrophyllus Seeds and Their Cytotoxic Activity against MCF-7 Breast Cancer Cell Lines

Dewa Gede Katja, Intan Rahmayanti, Nurlelasari, Siska Elisahbet Sinaga, Kansy Haikal, Al Arofatus Naini, Khalijah Awang, Unang Supratman
https://doi.org/10.48048/tis.2025.9173

Abstract

Meliaceae family consists of various plants that have been used as a source of medicinal plants and limonoid compounds. Limonoid is a compound group of terpenoid which has many bioactivities. One of the species from Meliaceae which produced limonoids is Chisocheton macrophyllus. The n-hexane fraction of the C. macrophyllus seed afforded 3 limonoids, namely 7-deacetylepoxyazadiradione (1), 6α-acetoxydeoxygedunin (2) and 6α-acetoxygedunin (3). Data from a variety of spectroscopic techniques, including as mass spectroscopy, FTIR, 1- and 2-dimensional NMR, were used to identify the secondary metabolite structures, and the PrestoBlue^TM, method was used for the cytotoxic assay. Compound (2), known as 6α-acetoxydeoxygedunin, was first reported in this genus. MCF-7 breast cancer cell lines have also been used to assess limonoids compounds (1 – 3). The 2 and 3 were categorized as weak with an IC₅₀ value of 231.26 and 475.87 µg/mL, while compound 1 showed inactive with IC₅₀ values of > 500 µg/mL. These findings encourage further exploration of Meliaceae plants for new bioactive compounds and advance the development of novel cancer treatments.

Keywords:

Cytotoxic activity; Chisocheton macrophyllus; MCF-7 cell lines; Limonoids