Isolation, Identification and Characterization of Gibberellin GA-3 Phytohormone from Dark Septate Endophyte (DSE) of Sunflower Helianthus annuus

Hajar Tsabitah, Safri Ishmayana and Ukun MS Soedjanaatmadja
http://dx.doi.org/10.31080/ASNH.2024.08.1356

Abstract

Dark Septate Endophyte (DSE) is a group of endophytic fungi that interact in symbiotic mutualism with their host plants and are beneficial in plant growth by facilitating the absorption of nutrients, protecting against biotic and abiotic stress and producing phytohormones such as gibberellins, auxins and cytokinins. This study aims to determine the gibberellin content contained in DSE isolated from sunflower roots (Helianthus annuus L.) from Tangerang, Banten-Indonesia, determine the effect of the gibberellin hormone in DSE in stimulating the growth of rice plants (Oryza sativa) in the vegetative phase and the activity of DSE isolates. against metal and salt stress. DSE inoculum was made on PDB media, analysis of gibberellin production on DSE using Thin Layer Chromatography, adsorption chromatography and High Performance Liquid Chromatography (HPLC), inoculation of DSE on rice seeds, detection of colonization in rice with a 400 times magnification microscope, and test of the activity of gibberellin isolates on vegetative phase rice growth. The activity of gibberellin isolates and DSE isolates was determined by bio-testing on rice plants (O. sativa) by measuring the length and dry weight of the plant shoots. In DSE isolates, gibberellin levels in the growth medium were found to be 0.561 mg/mL. The results showed that in the first week the canopy length increased with an active percentage of 188%. Based on the canopy dry weight parameter, the percentage of activity in the first week was 128.17%. DSE inoculation of rice plants with metal and salt stress can increase the growth tolerance of rice plants (O. sativa) in the vegetative phase based on the parameters of shoot dry weight and shoot length.

Keywords:

Dark Septate Endophyte (DSE); Gibberellin; H. annuus L; O. sativa