Home Penelitian Biokimia Development of Biostimulant for Improvement Productivity of Plants; Isolation and Formulation of Phytohormones as The Biostimulant

Development of Biostimulant
for Improvement Productivity of Plants;
Isolation and Formulation of Phytohormones as The Biostimulant


R. Ukun MS Soedjanaatmadja1*, Wening Astriani Susilo1, Hapsari Murdiani Putri1, Elizabeth Tampubolon1, Saadah Diana Rachman1, Reginawanti Hindersyah2 & Ace Tatang Hidayat1

1. Chemistry Department, Faculty of Mathematics and Natural Sciences Jl. Singaperbangsa No.2. Bandung 40133.
2. Agriculture Faculty , Jl Raya Bandung-Sumedang KM 21 Jatinangor

* Correspondence Author: This e-mail address is being protected from spambots. You need JavaScript enabled to view it ; This e-mail address is being protected from spambots. You need JavaScript enabled to view it


Abstrac, Phyto-hormone or plant hormone is an organic substance that produced by the plants and has the important role in the regulation process in the plants. The biochemical process in the plants is not apart from the role of synthesized phyto-hormones, and every plant has ability to produce the phyto-hormones itself (as the endogenous hormones), for the process and continuity of the plant normal growth. But for accelerating the growth of plant are needed the adding hormones (as the exogenous hormones.). Base of that, for accelerating and increasing the productivity of crops, especially for the food and horticulture plants are needed to add the exogenous phyto-hormones to the plant. Through the multi-years research activities had been done about the seeking of several of the potential natural phyto-hormone sources, as the materials for bio-stimulants formulation which could be applied in agriculture field. Beside the extraction, isolation and analysis of auxin from Phaseolis mungo, cytokinin from aqua Cocos nucifera, trans-zeatin from Zea mays, had been found also the several phyto-hormone sources as the potential source of auxin, cytokinin and gibberellin. The potential phyto-hormone sources were red algae Eucheuma cotonii, Gracilaria coronopifolia, and the fruit of Momordica charantia, respectively. Extraction, isolation and analysis of auxin from E. cotonii, and cytokinin from G. coronopifolia, through maceration process, extraction by using organic solvent and TLC, later on analysis by using a reversed phase HPLC on the ODS Nucleosil C-18 column, it was found that the auxin content in E. cotonii was 5.34 x 10-3 mg/g of dry weight of algae, and the cytokinin content in G. coronopifolia was 6.26×10-2 mg/g of dry weight of algae. Thus anyway, isolation and analysis of gibberrelin from the fruit of M. charantia through maceration process by using methanol solvent, extraction with ethyl acetate, TLC (analytical and preparative), and analysis using the reversed phase HPLC on the ODS Nucleosil C-18 column, it was found that the gibberrelin content in the fruit of M. charantia was 4.185 mg/g of wet weight of the fruit. Phyto-hormones (auxin, cytokinin and gibberellin) isolated from the several sources (P. mungo, Z. mays, C. nucifera, red algae E. cotonii and G. coronopifolia, and the fruit of M. charantia) were formulated by adding the other macro and micro nutrition (carbohydrates, proteins, essential amino acids, vitamins, antioxidants, and minerals from the various of natural sources), to become the Biostimulant ETAC-21 dan Biostimulant ETAC-12.

Keywords: Fitohormon, Eucheuma cotonii, Gracilaria coronopifolia, Momordica charantica, Biostimulan ETAC-21, Biostimulan ETAC-12.